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We also found that short-term CCCP exposure considerably increased the proliferation of MUC1-proficient cells in either MDA-MB-468 (Fig

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Two-step assay with fixation prior to chase step: - DBCOcl incubation, formaldehyde fixation, and azido-tagged dye DBCO-labeled cells prepared as described earlier were washed 3X with PBS and resuspended in 4% formaldehyde in PBS and incubated statically at room temperature on the benchtop for 30 min