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10.1164/rccm.202303-0491OC 48 FonsecaW.MalinczakC

Further, the SOE PCR products were cloned into FastDigest BshTI/FastDigest BglII (Thermo Fisher) linearized pEGFP-C2 vector using ClonExpress II One Step Cloning Kit (C112, Vazyme) to obtain the six sorting vectors, pEGFP-GST-GPI DAF , pEGFP-GST-GPI BY55 , pEGFP-GST-GPI CEAM7 , pEGFP-GST-TMD ITB3 , pEGFP-GST-TMD ITA5, and pEGFP-GST-TMD ITAV

Sensorgrams were analyzed using a global fit to the 1:1 Langmuir binding model

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In conclusion, our study shows that exogenous PAF C-16 can inhibit the intracellular growth of M