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HPLC Tissue preparation To measure the levels of endogenous DA and related metabolites DOPAC and HVA, striata from adult mice were homogenized in 0.092 M perchloric acid with an ultrasonicator (VibraCell, VCX 500, Sonics) for 10 s

Immediately, after the second day of reaccess to ethanol (after the alcohol reading at day 63, the animals were anesthetized with chloral hydrate (280 mg/kg, i.p.) and euthanized to obtain hippocampal samples

However, Ca 2+ -channel blockers have failed to become an established treatment for bipolar disorder as there is mixed evidence on their efficacy, which perhaps argues that the observed increase in free intracellular Ca 2+ in patients may simply be a marker of the underlying disturbance rather than causative [25]

2,3 Food sources of glutathione, however, comprise only a small amount of the bodys total glutathione levels, since most is endogenously synthesized at the cellular level by the body itself