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Since many pathologic and toxic conditions result in sustained increase in cytosolic free Ca 2+ , which can lead to activation of CMK and phosphorylation of GCLC, theoretically, inhibition of GCL may further contribute to toxicity under these conditions

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Consistently, XKR8 KO neutrophils released significantly less extracellular double-stranded DNA (dsDNA) in the culture medium compared to WT controls during NET formation (Extended Data Fig