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Protein from the 21NT, BT474, HMEC and IBR.3 cells were extracted using the TRAPeze 1 CHAPS lysis buffer (S7705, Millipore) and quantified using the CB-X protein assay kit (G-Bioscience)

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Many drugs used to treat obesity in the past have been discontinued because of their potential to be abused and their toxicities [20-27] (Table 1 )

Another issue is timing