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Thats why they must be provided through the diet

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Furthermore, accumulating evidence underscores the involvement of the gut microbiome in the aetiology of NAFLD through the mediation of NAFLD metabolites, such as trimethylamine N-oxide (TMAO) (Reference Chen and Vitetta7) , which is naturally found in seafood, dairy products, egg yolks, muscle and organ meats in a preformed state and is also a metabolite originating from precursors, including phosphatidylcholine, choline, betaine and l-carnitine (Reference Thomas and Fernandez8,Reference Li, Lu and Yuan9)

For saline treatment, mice were injected intraperitoneally once per day on postnatal days 1428, along with standard mouse feed pellets and drinking water, followed by normal pellets and drinking water