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Horseradish peroxidase-conjugated secondary antibodies and an enhanced chemiluminescence substrate kit were used for the detection of specific proteins

Moreover, in their study, weight loss increased GH secretion but did not affect ghrelin levels (523)

doi: 10.1002/ijc.29095

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This means it has not undergone FDA review for safety, efficacy, or manufacturing quality , and it cannot be marketed or sold legally as a dietary supplement or medication for human consumption