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Treatments comprised 10 mM glucose, 1 M oligomycin and 50 mM 2-deoxy-d-glucose (BioVision, B1048-100)

10.1016/j.kint.2020.06.019 66 Kidney Disease: Improving Global Outcomes (KDIGO) Diabetes Work Group (2022)

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Spheroid formation was assayed by microscopy (Zeiss Axio A1 inverted microscope) 57 days after seeding and registered using a CANON Rebel T3i camera attached to the microscope with an appropriate adaptor, at 40 magnification