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Samples that did not correctly inject, judged by lack of signal for the internal standard, on the MS were also omitted from analysis

doi: 10.1080/07853890.2023.2209335

Gut bacteria regulate neurotransmitters, influencing mental health and brain function

Intestinal enzymes can break down neither oligosaccharides nor polysaccharides

2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions

To perform Na + dependence assays, carnitine doses were applied to oocytes expressing hOCTN2, and related mutants, in the presence of Cl - buffers of differing Na + concentration (10100 mM NaCl, 100 mM - [NaCl] CsCl, 2 mM KCl, 1 mM MgCl 2 , 1.8 mM CaCl 2 , 5 mM HEPES, buffered to pH 7.4 with Tris base)