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The dispersed distribution of the scaffold-type proteins, particularly those characterized as the carrier or transfer proteins, is important for the determination of the mechanistic specificity of the FeS cluster assembly systems
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In the presence of iron ions, superoxide acquires a second electron, leading to hydrogen peroxide formation, which generated the extremely reactive hydroxyl radical
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They were then spun down, removed of their supernatant, and resuspended and washed with several changes of PBS, then washed over night in 5 mL PBS
doi: 10.1016/j.cell.2022.06.003 70 BouchaouiHMahoney-SanchezLGaronGBerdeauxOAllemanLYDevosDet al
