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During exercise, the muscle signals its energy requirements to the liver by changing the concentration of acylcarnitine in the blood, and the liver adjusts its metabolic activity to increase the synthesis and release of energy substances [223, 224], support muscle contraction and meet the energy demands of exercise [225, 226]

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To determine whether these proteins associate with the luminal or cytosolic side of the OMM or are embedded within the OMM, we incubated purified mitochondria with trypsin