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Briefly, fecal samples were suspended in sterile distilled water by vigorous shaking, and the supernatant was transferred to a 0.5 ml tube containing glass beads (0.1 mm diameter), before being spun at 5,000 rpm for 3 min in a mini-bead beater (BioSpec Products, Bartlesville, OK, USA)

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212 In patients with headache ( n = 31), including migraine and other types of chronic headache, 5-HTP (400 mg daily) for 2 months showed that 48% of participants had a greater than 50% average reduction in headache symptoms

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