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Lyse cells by sonication on ice (~10 times for 10 sec each with 1 min rest between bursts to minimize sample heating)
[DOI] [PubMed] [Google Scholar] 40.Keremi B., Lohinai Z., Komora P., Duhaj S., Borsi K., Jobbagy-Ovari G., Kallo K., Szekely A.D., Fazekas A., Dobo-Nagy C., et al
Because NAC is known to be a very effective free radical scavenger it was used to test this theory
doi: 10.1038/nrd1066
10.26444/jpccr/81277 Summary Keywords oxidative stress, reactive oxygen species, reactive nitrogen species, free radicals, antioxidants, lipid peroxidation Citation Krishnamurthy HK, Pereira M, Rajavelu I, Jayaraman V, Krishna K, Wang T, Bei K and Rajasekaran JJ (2024) Oxidative stress: fundamentals and advances in quantification techniques
On the other hand, heat stress increased SOD and decreased GSH-Px activities of mitochondria regardless of muscle types [120]
