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However, studies have also demonstrated that, unlike the 2 -AR, this subtype did not internalize into intracellular vesicles upon agonist stimulation (Toews, 1987)

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Typical volume: 0.1-0.2 mL with a 29-31 gauge insulin syringe

After replacing the special test medium with the hippocampal medium, the culture was incubated in a non-CO 2 incubator for 1 h