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Similar to pharmacologic inhibition of GSH synthesis, loss of GCLC, the rate limiting enzyme of GSH synthesis, also strongly induced SLC25A39 protein (Fig

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The magnitude of Chac1 downregulation exceeded that of canonical refeeding-responsive genes controlling hepatic glucose metabolism, such as the downregulation of G6pc or upregulation of Gck (Figure 6A), indicating robust transcriptional suppression of Chac1 during feeding