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During the pre-stroke phase, sudden brain hypoxia damages the BBB, causing neuroinflammation, cytotoxic edema, production of reactive oxygen species, breakdown of tight junctions and the extracellular matrix, and increased BBB permeability

Dr Linsemans approach is to minimise the damage caused by ROS in the injured brain

This test is a non-specific measure of inflammation in the body

Most supporting research comes from animal and cell studies, including tendon and ligament injury models, while large human trials have not been identified

On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin

Zinc contributes critically to this antioxidant defense as a structural and catalytic cofactor for Cu/Zn-superoxide dismutases (SODs), which convert superoxide radicals into hydrogen peroxide 93