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In this test, each mouse was habituated to the test cage for 1 min before being held by the scruff

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 24:185187 Noland RC, Koves TR, Seiler SE, Lum H, Lust RM, Ilkayeva O, Stevens RD, Hegardt FG, Muoio DM (2009) Carnitine insufficiency caused by aging and overnutrition compromises mitochondrial performance and metabolic control

Conjugated proteins were detected by an enhanced chemiluminescent immunoblotting detection system, the ChemiDoc Touch Imaging System (BIO RAD, CA, USA) was performed

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Int J Biometeorol