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How to Safely Administer B12 Shots at Home Self-administering B12 injections at home requires proper training, technique, and adherence to safety protocols

2.2 Laboratory-scale bioreactor cell culture At the laboratory scale, MRC-5 cells were cultured in 3 L glass bioreactors with a BioFlo 320 controller (Cat#: 1379962911, Eppendorf, Hamburg, Germany) on 1 g/L Cytodex 1 microcarriers (Cat#: 17548702, Cytiva, Marlborough, MA) or in iCELLis Nano fixed-bed bioreactors (Pall Corporation, Port Washington, NY) with a 10 cm bed height with WMEM and 10% BCS controlled at a target temperature of 37C and pH of 7.2 as described previously (Swartz et al., 2022)
doi: 10.3390/agriculture2040452 30

doi: 10.1007/s00702-006-0485-7

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In de novo hypothyroid adults without cardiac disease, total daily doses of 20-25 mcg divided two or three times achieve physiologic free T3 levels for most patients
