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Systematic mapping of proteinmetabolite interactions in central metabolism of Escherichia coli

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Double Immunolabeling Following first primary antibody, tissue was subsequently blocked in 5% normal horse serum, followed with separate blocking steps in streptavidin and biotin solutions (from ABC kit) five minutes each before second primary antibody reaction

1c and Supplementary Table 3)

We could do this because the gdh3 / gdh3 mutant was unable to undergo morphogenesis in the proline medium but became highly filamentous in the arginine medium (Fig