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The cells were mixed well with antibodies and incubated in a darkroom at room temperature for 15 min

However, further research is essential to fully comprehend the interactions between cerebellar microglia and infiltrating immune cells Early activation of cerebellar microglia has also been observed in other lysosomal storage disorders, such as GM2 gangliosidosis (Tay-Sachs disease), mucopolysaccharidosis type IIIB, infantile neuronal ceroid lipofuscinosis, and claudin 7 (Cln7) deficiency (Macauley et al., 2009

(2021b) identified that the fertilization effect of H 2 resulted in the proliferation of plant-growth-promoting rhizobacteria, and the enhancement of plant nutrient uptake and use efficiency, indicating that H 2 has the potential to be an alternative fertilizer to some extent and, in turn, reducing the use of chemical fertilizers (Li et al., 2021b) (Figure 2)

The reconstitution process itself is straightforward, but accuracy matters

Bacterial cultures were collected when the optical density (OD) at 600 nm reached 0.4, washed with PBS and prepared for subsequent experiments

Sweet potato has agronomical importance as the worlds 7th most economically important starch crop (global production of 93.5 million metric tons annually, and China producing 55% of global output alone) FAO, 2023, Previous studies on sweet potato under heat stress have primarily focused on gene expression, storage root development, and genetic transformation, with limited research on vegetable sweet potatoes [28,29,30,31], and also the absence of previous nanomaterial-based stress mitigation studies in this species under heat stress