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These findings may suggest that the genetic or pharmacologic overload of intraorganellar AA might interfere with the metabolism or traffic of BA metabolite(s) which impairs the protection conferred by LROs against BA toxicity, which remains to be characterized
To achieve GSH depletion in liver, we engineered an adeno-associated virus (AAV) vector encoding Chac1 fused to a Myc tag, driven by the thyroxine-binding globulin (TBG) promoter to achieve liver-specific overexpression of Chac1 (Figure 1B)
These samples were used for subsequent biochemical experiments
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