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Absorbance at 610 nm was recorded on a plate reader (Synergy, BioTek)

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However, in the case of selenoprotein messenger ribonucleic acid (mRNA), the 3untranslated region contains selenocysteine insertion sequence (SECIS) elements, which allow for the recognition of UGA as a selenium cysteine codon rather than a stop signal (21)
[PubMed Citation] Ristikankare A, Kuitunen T, Kuitunen A, Uotila L, Vento A, Suojaranta-Ylinen R, Salmenpera M, Poyhia R

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