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2 mM 2-mercaptoethanol, 0.01% NP-40) for two times, uS10 was eluted from beads by incubation with 600 L of Elution Buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl, 10 mM MgCl 2 , 300 mM Imidazole

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