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Delayed treatment may fail to mitigate the oxidative cascade, while early intervention could interfere with beneficial immune responses that rely on ROS for pathogen clearance (Forman and Zhang, 2021)

Methods Vectors All cloning was done in DH5alpha E

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For instance, Teh et al

Blockade of GCH1/BH4 axis activates ferritinophagy to mitigate the resistance of colorectal cancer to erastin-induced ferroptosis

In vivo yeast expression assays of the LjFNSII and LmFNSII proteins To investigate the catalytic activity of the enzymes encoded by the isolated putative FNSII genes, the coding region of each sequence was inserted into the pYeDP60 vector 25 and transformed into the WAT11 yeast strain, which expresses the Arabidopsis NADPH-cytochrome P450 reductase 26 and provides the reducing equivalents indispensable for the activity of plant CYP450s such as flavone synthase II enzymes