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High cortisol levels can lead to depletion of glutathione, a critical antioxidant involved in the detoxification of harmful substances

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[DOI] [PMC free article] [PubMed] [Google Scholar] 72.Saisawang C., Wongsantichon J., Robinson R.C., Ketterman A.J

The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2