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After 24 h, viability was assessed using the AquaBluer assay as previously described ( Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C

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Leu1 and aconitase enzyme assays The pellet of yeast cell cultures was suspended in lysis buffer (TrisHCl (10 mM, pH 7.4), EDTA (2.5 mM), NaCl (150 mM), glycerol (10%, v/v) and Triton X100 (0.5%, v/v)), and lysed by agitation with glass beads