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First, VSMC-derived sEVs were identified by western blot, NanoSight tracing analysis, and transmission electron microscopy (TEM), in which sEVs were positive for exosome protein markers (CD63 and CD81) with approximate diameters 80% cellular ATP in VSMCs
Interact with neurotransmitter systems , possibly affecting serotonin, dopamine, and GABA signaling pathways

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The interactions between NNMT and three methionine cycle-related proteins AHCY, BHMT, and MAT1A were validated through Co-IP followed by immunoblotting in mouse liver and 293 T cells [40]

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