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In brief, 100 L of the diluted plasma samples (1:100) was incubated on a 96-well microplate for 1 h at RT

Simultaneous Saccharification and Fermentation SSF was carried out as described by Ask et al

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This 3 mg/kg dose of TRV130 showed comparable respiratory depression to 8 mg/kg of morphine, which is similar to what was initially reported [19] but is higher than that shown in another recent report, which that measured analgesia with the hot plate assay [20]