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Do not chew or crush

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The bacteria were then washed twice with M9 to remove FAs and growth media, diluted to equal OD600, concentrated 10X for PA and 20X for EPA by centrifugation, resuspended in M9, and seeded onto NGM plates lacking peptone (200 ml/plate)

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(2021) Nazi Blockade of the Caribbean in 1942: Effects on Puerto Rico. Encyclopedia of Puerto Rico, June 27, 2021
