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All controlled studies referenced below were conducted in cell culture (3T3-L1 adipocytes, primary muscle stem cells) or rodent models (diet-induced obese mice, aged C57BL/6J mice)
10.1017/s1368980017001768 [DOI] [PMC free article] [PubMed] [Google Scholar] 25.Loizou S, Paraschos S, Mitakou S, et al
(PubMed) Bailey RL, Looker AC, Lu Z, et al

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Clinical guidance for intramuscular injections points to a 22 to 25 gauge needle, given at a 90-degree angle, with the length chosen so it reaches muscle without going too deep
