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Membranes were blocked with 5% (w/v) milk powder in PBS for 1 h and incubated for 16 h at 4 C with one of the following primary antibodies: rabbit anti-SLC31A1/CTR1 (Abcam, Cambridge, UK), mouse anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-XPF (Thermo Fisher, Waltham, MA, USA), mouse anti-ERCC1 (Santa Cruz Biotechnology), mouse anti-Tubulin (Abcam), rabbit anti-NRF2 (Abcam)

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This protection ensures ATP production continues efficiently, which directly supports energy levels, muscle recovery, and overall performance