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Disrupted fronto-parietal network and default-mode network gamma interactions distinguishing suicidal ideation and suicide attempt in depression

2008 Aug;93(8):3075-81

On day 20, cells were dissociated using Accutase (Merck, Kenilworth, NJ), re-plated at 0.75 10 5 cells per cm 2 on dishes pre-coated with polyornithine (15 g/ml), laminin (1 g/ml) and fibronectin (2 g/ml), and cultured in neurobasal medium with 2% B27 without vitamin A and with 1% penicillin/streptomycin and trophic factors (GDNF, BDNF, TGFb3, AA, db-cAMP and DAPT) until analysis
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To address this issue and provide steady, continuous delivery of GLP-1RAs, other controlled-release devices such as osmotic pumps have been explored
