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40 L of SYBR Gold solution prepared above was added to each well (final dilution 1:10,000) and fluorescence was measured with a 96well fluorometer (PerkinElmer, USA) at an emission wavelength of 535 nm and an excitation wavelength of 485 nm

doi: 10.3389/fimmu.2018.00036

Materials and Methods Cell Culture 3T3 fibroblasts were cultured in Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (Invitrogen, San Diego, CA, USA) and antibiotics (25 U/ml penicillin, 25 g/ml streptomycin, and 0.3 g/ml amphotericin B) (Invitrogen, San Diego, CA, USA) in 5% CO 2 in air at 37C in 25 or 75 cm 2 flasks

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Additionally, RNA interference was employed to examine the effects of these two genes on a range of physiological characteristics of B

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