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HPLC Parameters: 1) Column Operating Temperature (Procedural temperature is 30C) a) Temperature Plus (+5C) 35C b) Temperature Minus (5C) 25C 2) Buffer Mobile Phase (Procedural pH 2.60) a) pH Minus (0.2) pH 2.40 b) pH Plus (+0.2) pH 2.80 Derivatization Procedure Parameters: Six (6) derivatized sample solutions were prepared in duplicate by changing the derivatization procedure parameters such as reaction time, reaction temperature, and concentration of Solution-3, and they were poured into the chromatographic apparatus in accordance with the protocols guidelines

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