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Description
A critical event for mitochondrial apoptosis is the release of CYCS or AIFM1/AIF (apoptosis inducing factor mitochondria associated 1) from the mitochondria, which mediates apoptosis through activating caspases (e.g., CASP9 [caspase 9]) or endogenous endonucleases, respectively [75,76]
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Samineni VK, Premkumar LS, Faingold CL
The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)
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