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Draw the Diluent: Pull back the plunger on your syringe to the desired volume mark (e.g., 100 units for 1mL)

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When using dilute acetic acid (0.1% v/v) as a solvent, it can improve initial solubilization of certain peptide batches, but the solution pH should be adjusted to near-physiological range before administration to avoid tissue irritation at the injection site in animal models
Endotoxicity This test, performed alongside sterility testing, ensures the medication is free from harmful bacterial toxins

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