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Isolation of polyribosomes containing actively translating mRNAs Using a modified method from Cleuren et al

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Discussion Here, we use microdomain-targeted expression of the H 2 O 2 biosensor HyPer7 and ROS photogenerator KillerRed to measure the kinetics of ROS generation and diffusion throughout mitochondrial microdomains

The reaction was started with addition of an equal volume of DTNB (6 mM) and NADPH (100 M) and the increase in A 412 during the first 3 min was recorded