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Treating femoral nerve entrapment requires incorporating several different approaches the 4 Rs (check out my article on the 4 Rs)

The sample preparation protocol is based on the simultaneous reduction with TCEP of disulfide bonds and derivatization of thiol groups with BCPB and the deproteinization of the mixture with PCA

References Abreu R.V., Silva-Oliveira E.M., Moraes M.F.D., Pereira G.S., Moraes-Santos T

Cytoplasmic inheritance of chloramphenicol resistance in mammalian cells

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