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Yeast strains The following yeast strains were used: SP4, a wild-type strain with the genotype MAT, leu1, arg4 70 , referred to here as wt1 DSCD1-1C, a mutant derived from the parent strain SP4 with the genotype MAT, leu1, arg4, sod1::natMX 25 , referred to as sod1 EG103, a wild-type strain with the genotype MAT leu2-3,112 his3l trp1-289 ura3-52 , referred to here as wt2, and its derivative strains containing coding region insertions TRP1 in EG110 ( sod2 ::TRP1), referred to as sod2

Refolding of the DAM27 protein was achieved by dialysis into aqueous buffer directly using the desulfurization reaction without a need of HPLC purification due to complete conversion (Figs

These results suggest that similar metabolic alterations occur in murine and human kidneys with TLS and that urinary GSH could be a noninvasive diagnostic biomarker to detect TLS in the kidney

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For lean individuals, particularly those with muscular thighs from training, the margin between subcutaneous and intramuscular injection narrows

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