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After washing three times with PBST again, the membranes were incubated with the secondary antibody for 1 h at room temperature

The pump was kept running at 70 L/min using 50% ACN as a pushing solvent at isocratic pressure before the experiments to ensure a stable flow of the solvent

By doing this, we can help create an environment where everyone thrives both personally and professionally

Further research has shown that S100B levels are correlated with increased ICP in both the early and late phases following TBI

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