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PMID: 16996037), a single intranasal application of Semax at 50 g/kg in rats produced a maximal 1.4-fold increase in hippocampal BDNF protein levels, a 1.6-fold increase in TrkB tyrosine phosphorylation, a 3-fold increase in exon III BDNF mRNA, and a 2-fold increase in TrkB mRNA effects observed within hours of a single administration

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The fold should feel soft and mobile, confirming you have grasped fat rather than muscle