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Cells were grown to ~70% confluency before treatment for 24 hours with various concentrations of bovine intestinal alkaline phosphatase (Sigma: A2356) in the absence or presence of bafilomycin A1, an autophagy flux inhibitor (100 nM, added for 3 hrs towards the end of 24 hr incubation.) (Sigma: SML1661)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 35.Tomasova L., Konopelski P., Ufnal M

Risteli et al ., 2004

Reducing frequency and pausing other actives usually resolves the breakouts within a month

6,350,467, references the use of glutathione and ascorbic acid to influence the redox status of cells in disease states, however does not reference the use of a liposome encapsulation to deliver and maintain the glutathione in the reduced state to the system