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Amplicon sizes were verified by 1.5% agarose gel electrophoresis

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Adv Drug Deliv Rev 64:640665 Kannan R, Kuhlenkamp JF, Ookhtens M, Kaplowitz N (1992) Transport of glutathione at blood-brain barrier of the rat: inhibition by glutathione analogs and age-dependence

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The Q Switch Nd:YAG laser operates at two energy frequencies [4] : 1064 nm which is used to target deeper skin layers 532 nm , which is used to address hyperpigmentation and more superficial layers
