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Cell Culture and GSH Preparation K562, HepG2, Hela and L-02 cells were respectively cultured in RPMI 1640 (Hyclone, penicillin 100 U/mL, streptomycin 100 g/mL) adding 10% fetal bovine serum and maintained at 37 C in a humidified atmosphere including 5% CO 2 , in terms of the instructions of the American Type Culture Collection

[Google Scholar] [206].Zhang Y, Leonessa F, Clarke R, Wainer IW, Development of an immobilized P-glycoprotein stationary phase for on-line liquid chromatographic determination of drug-binding affinities, J

Yeast cultures (10 mL) were grown in 50 mL Erlenmeyer flask from single colonies at 30 C, 120 rev

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[DOI] [PubMed] [Google Scholar] 20.Nawarskas J.J

Binding analysis of methyl-cpG binding domain of meCP2 and rett syndrome mutations