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34 shows the BDOC variation of each BCF bed section in this period

Using finer gauge needles (29-31 gauge), inserting at a 45-degree angle initially before going vertical, and rotating slightly during insertion all reduce coring risk

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BT474 cells and PC3- hTERT cells were cultured in DMEM F-12 medium (Gibco, Invitrogen) with 10% FBS (Gibco, Invitrogen) and 1% PenicillinStreptomycin (Gibco)
