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FIGURE 3 To verify that these phenomena were indeed caused by GPX4 activation, we tested the effects of compound 102 on other seven enzymes in the AA network, including COX-1, COX-2, mPGES-1, 5-LOX, LTA 4 H, 12-LOX, and 15-LOX

RMA tracing for untargeted metabolite identification 13 C 15 N-cystine tracing Cells seeded for intracellular metabolite extractions, as described above, and changed to assay medium (DMEM) containing dialysed FBS and a ~1:1 ratio of labelled:unlabelled cystine, accomplished by adding 200 M 13 C 6 15 N 2 L-cystine (Cambridge Isotopes, CNLM-4244-H-PK) to the medium

doi: 10.1038/nrgastro.2017.136 156 LevineASigall BonehRWineE

As previously described, the roGFP probe is already partially oxidized under standard temperature conditions, which is due to a lower glutathione redox potential (Figure 3, Meyer et al., 2007)

Idratazione cellulare e funzione muscolare: La taurina agisce come osmolitico organico ed coinvolta nella regolazione del calcio, entrambi fattori rilevanti per la contrazione muscolare e la gestione dell'affaticamento