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The HPLC was set at a flow rate of 0.25 ml min 1 with 99% buffer A from 0 to 3 min, a linear gradient to 99% buffer A/1% buffer B (100% methanol) from 3 to 20 min, 80% buffer A/20% buffer B from 20 to 21 min, a linear gradient to 30% buffer A/70% buffer B from 21 to 28 min at 0.35 ml min 1 , 99% buffer A/1% buffer B from 28 to 31 min and a linear gradient to 99% buffer A from 31 to 37 min at 0.25 ml min 1

The molecule has been studied in animal models of adipose-tissue metabolism, with published preclinical work examining its effects on NAD+ pool dynamics and on the methyl-donor balance NNMT regulates

In a 23-week randomized clinical trial in humans, individuals taking AOD-9604 at 1 mg daily had an average of 2.8 kg weight reduction compared to a 0.8 kg weight reduction in those taking a placebo

Front Endocrinol, 2022 Human trials (AOD-9604): Safety/tolerability reported across multiple RCTs

The NAD+ precursor nicotinamide riboside rescues mitochondrial defects and neuronal loss in iPSC and fly models of Parkinsons disease