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In keeping with the selection of high GSH producers which should have a reduced pGSH1 expression level, the GAP1 mRNA level was markedly lower during cultivation with D-HIS selection (Fig

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Neuroreport 7 : 21812185

After washing five times with a buffer containing 20 mM HEPES pH 7.0, 150 mM NaCl, and 2 mM -ME, the resin was incubated with the cell lysates containing bait protein overnight at 4 C