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Some models take already existing PDMCTs and develop a new model from them, such as bioprinted material established from patient-derived cell lines 162 and organotypic cultures established from patient-derived xenografts 163

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Once the culture has been induced with IPTG, do not allow the cells to grow for extended periods of time, as cell lysis can occur and release proteases into the cytoplasm that could degrade the fusion protein

doi: 10.1016/j.bios.2020.112435 194 MittalM.SiddiquiM

The bioactive compounds in cardamom, such as limonene, caffeic acid, and cineole, downregulate several signaling pathways in cancer cells by inhibiting cyclooxygenase-2 and cytochrome P450 [37]