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For intramuscular injection, stretch the skin taut and insert the needle at 90 degrees into the muscle

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As per a study conducted, it was found that nearly 90% of people are deficient in Vitamin -12

For example, Tai et al., evaluated the antioxidant activity of vanillin by using multiple antioxidant assays and the experimental results indicated that vanillin exhibited a potent antioxidant effect in 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical cation/ABTS + scavenging assay, oxygen radical absorbance capacity (ORAC) assay and oxidative hemolysis inhibition assay (OxHLIA) (Tai et al., 2011)

Furthermore, they only look at serum B12 as a biomarker of B12 deficiency and do not consider methylmalonic acid, homocysteine, methionine, haematological parameters, or clinical symptomatology in their assessment of therapeutic effect

Then, in 2015, Lee and colleagues identified a previously overlooked open reading frame within the 12S ribosomal RNA gene of mtDNA and found that it encodes a functional peptide, MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA-c) [1]